Proton turnover rates of single heme copper oxidases operating against electrochemical gradients

Principal Investigators: Dr. Stephan Block (FU)

In this project heme copper oxidases (cytochrome c oxidase and ubiquinol oxidase) are reconstituted into liposomes and advanced microscopic approaches are applied to quantify the proton turnover rate of single oxidases based on recording the pH changes occurring within the proteoliposomes. The dynamics of proton uptake by the oxidases will be quantified in dependence of the generated electrochemical gradient. By these means, we will achieve an understanding of the regulation of proton pumping by the electrochemical gradient.

Publications

2017-2020

Berg, J., Block, S., Höök, F., and Brzezinski, P. (2017). Single proteoliposomes with E. coli quinol oxidase: proton pumping without transmembrane leaks. Isr. J. Chem. 57, 437-445; doi: 10.1002/ijch.201600138

Friedrich, R., Block, S., Alizadehheidari, M., Heider, S., Fritzsche, J., Esbjörner, E., Westerlund, F., and Bally, M. (2017). A nano flow cytometer for single lipid vesicle analysis. Lab Chip 17, 830-841; doi: 10.1039/c6lc01302c

Hugentobler, K., Heinrich, D., Berg, J., Heberle, J., Brzezinski, P., Schlesinger, R., Block, S. (2020) Lipid Composition Affects the Efficiency in the Functional Reconstitution of the Cytochrome c Oxidase. Int. J. Mol. Sci. , 21(19), 6981; https://doi.org/10.3390/ijms21196981

Müller, M., Lauster, D., Wildenauer, H.H.K., Herrmann, A., and Block, S. (2019). Mobility-based quantification of multivalent virus-receptor interactions: New insights into influenza A virus binding mode. Nano Lett. 19, 1875-1882; doi: 10.1021/acs.nanolett.8b04969

Parveen, N., Rydell, G. E., Larson, G., Hytönen, V. P., Zhdanov, V. P., Höök, F., and Block, S. (2019). Competition for membrane receptors: norovirus detachment via lectin attachment. J. Am. Chem. Soc. 141, 16303-16311; doi: 10.1021/jacs.9b06036

2016

Block, S., Johansson Fast, B., Lundgren, A., Zhdanov, V.P., and Höök, F. (2016). Two-dimensional flow nanometry of biological nanoparticles for accurate determination of their size and emission intensity. Nat. Commun. 7, 12956; doi: 10.1038/ncomms12956

Block, S., Zhdanov, V.P., and Höök, F. (2016). Quantification of multivalent interactions by tracking single biological nanoparticle mobility on a lipid membrane. Nano Lett. 16, 4382-4390; doi: 10.1021/acs.nanolett.6b01511