The SFB 1078 kindly invites you to the following colloquium talk:

Dr. Chen Song hosted by Prof. Jon Hughes (JLU):

Title: Solid-state NMR on trehalose-embedded phytochromes

Phytochromes represent a diverse family of photoreceptors that enable plants and microorganisms to adapt to changes in ambient light environment. Common to all phytochromes is the temperature-regulated thermal reversion. Despite methodological advances, short-lived photoproducts of phytochromes remain challenging for biophysical techniques such as solid-state NMR that require repetitive measurements due to rapid thermal reversion. To inhibit this light-independent reaction, we recently developed a simple method for photoproduct stabilisation by incorporating the protein into amorphous trehalose glasses (TGs). The resulting trehalose matrices exhibit the outstanding efficacy in this regard for long periods of time (weeks) at room temperature. More importantly, the advantages of trehalose–protein glassy matrices also allow to isolate individual transient reaction intermediates and to measure them separately under non-cryogenic conditions. In this talk, I will provide an overview of our recent solid-state NMR results of the TG-trapped photocycle states of a phytochrome-related cyanobacteriochrome which reflect changes in the local electronic structure, geometry, dynamics, and charge distribution of the chromophore during the photocycle.